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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
29/01/2024 |
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Data da última atualização: |
29/01/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
OLIVEIRA, C. S.; SARAIVA, N. Z.; OLIVEIRA, L. Z. |
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Afiliação: |
CLARA SLADE OLIVEIRA, CNPGL; NAIARA ZOCCAL SARAIVA, CNPGL; LETICIA ZOCCOLARO OLIVEIRA, UNIVERSIDADE FEDERAL DE MINAS GERAIS. |
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Título: |
Morphology of 16-cell embryo in bovine: Inside cells, compaction, fragmentation and effects of X-sorted semen. |
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Ano de publicação: |
2024 |
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Fonte/Imprenta: |
Anatomia, Histologia, Embryologia, v. 53, e13015, 2024. |
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DOI: |
https://doi.org/10.1111/ahe.13015 |
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Idioma: |
Inglês |
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Conteúdo: |
In mouse embryos, inside cells are allocated in 16-cell embryos through a well-orchestrated sequence of events involving compaction and polarization. The emergence of inside cells is of great importance as itl later gives rise to the inner cell mass and epiblast. In this study, we report the sequence of critical events in embryology (compaction, inside cells allocation and fragmentation) in bovine 72 h.p.i. 9-16 cell embryos, while also investigating the effects of X-sorted semen on these events. We found a wide distribution of total cell numbers among embryos, attributed to an asynchronous cleavage pattern and blastomere death. Additionally, 13% of embryos displayed irregular shapes. The establishment of the inside cell compartment increased (p < 0.01) in embryos with more cells. However, only 53.8% of 16-cell embryos presented inside cells. Compaction was present in 32.4% embryos and was positively correlated (p = 0.03, OR 3.02) with the establishment of inside cells, occurring independently of cell number. Fragmentation was present in 36% embryos, being more frequent (p = 0.01) in embryos with lower cell numbers. A possible association between irregular shape and fragmentation was considered (p = 0.06). The use of X-sorted semen had no effect on most evaluated parameters. However, it did have a marked effect on cleavage rate (p < 0.01) and the arrest of 2- and 4- cell embryos. In conclusion, bovine embryos exhibit an asynchronous cleavage pattern, high levels of fragmentation, and demonstrate compaction and inside cell allocation later in development compared to mouse embryos. Semen X-sorting has major effects on cleavage and embryo arrest. Further studies are needed to elucidate the association between irregularly shaped embryos and fragmentation, as well as the effects of sex on inside cell allocation. MenosIn mouse embryos, inside cells are allocated in 16-cell embryos through a well-orchestrated sequence of events involving compaction and polarization. The emergence of inside cells is of great importance as itl later gives rise to the inner cell mass and epiblast. In this study, we report the sequence of critical events in embryology (compaction, inside cells allocation and fragmentation) in bovine 72 h.p.i. 9-16 cell embryos, while also investigating the effects of X-sorted semen on these events. We found a wide distribution of total cell numbers among embryos, attributed to an asynchronous cleavage pattern and blastomere death. Additionally, 13% of embryos displayed irregular shapes. The establishment of the inside cell compartment increased (p < 0.01) in embryos with more cells. However, only 53.8% of 16-cell embryos presented inside cells. Compaction was present in 32.4% embryos and was positively correlated (p = 0.03, OR 3.02) with the establishment of inside cells, occurring independently of cell number. Fragmentation was present in 36% embryos, being more frequent (p = 0.01) in embryos with lower cell numbers. A possible association between irregular shape and fragmentation was considered (p = 0.06). The use of X-sorted semen had no effect on most evaluated parameters. However, it did have a marked effect on cleavage rate (p < 0.01) and the arrest of 2- and 4- cell embryos. In conclusion, bovine embryos exhibit an asynchronous cleavage pattern, high levels of fragmenta... Mostrar Tudo |
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Palavras-Chave: |
Compactação; Fertilização in vitro; Fragmentação; Massa celular interna. |
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Thesagro: |
Bovino; Embrião Animal; Reprodução Animal. |
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Thesaurus Nal: |
Embryo (animal). |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1161417/1/Morphology-of-16-cell-embryo-in-bovine.pdf
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Marc: |
LEADER 02636naa a2200253 a 4500
001 2161417
005 2024-01-29
008 2024 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1111/ahe.13015$2DOI
100 1 $aOLIVEIRA, C. S.
245 $aMorphology of 16-cell embryo in bovine$bInside cells, compaction, fragmentation and effects of X-sorted semen.$h[electronic resource]
260 $c2024
520 $aIn mouse embryos, inside cells are allocated in 16-cell embryos through a well-orchestrated sequence of events involving compaction and polarization. The emergence of inside cells is of great importance as itl later gives rise to the inner cell mass and epiblast. In this study, we report the sequence of critical events in embryology (compaction, inside cells allocation and fragmentation) in bovine 72 h.p.i. 9-16 cell embryos, while also investigating the effects of X-sorted semen on these events. We found a wide distribution of total cell numbers among embryos, attributed to an asynchronous cleavage pattern and blastomere death. Additionally, 13% of embryos displayed irregular shapes. The establishment of the inside cell compartment increased (p < 0.01) in embryos with more cells. However, only 53.8% of 16-cell embryos presented inside cells. Compaction was present in 32.4% embryos and was positively correlated (p = 0.03, OR 3.02) with the establishment of inside cells, occurring independently of cell number. Fragmentation was present in 36% embryos, being more frequent (p = 0.01) in embryos with lower cell numbers. A possible association between irregular shape and fragmentation was considered (p = 0.06). The use of X-sorted semen had no effect on most evaluated parameters. However, it did have a marked effect on cleavage rate (p < 0.01) and the arrest of 2- and 4- cell embryos. In conclusion, bovine embryos exhibit an asynchronous cleavage pattern, high levels of fragmentation, and demonstrate compaction and inside cell allocation later in development compared to mouse embryos. Semen X-sorting has major effects on cleavage and embryo arrest. Further studies are needed to elucidate the association between irregularly shaped embryos and fragmentation, as well as the effects of sex on inside cell allocation.
650 $aEmbryo (animal)
650 $aBovino
650 $aEmbrião Animal
650 $aReprodução Animal
653 $aCompactação
653 $aFertilização in vitro
653 $aFragmentação
653 $aMassa celular interna
700 1 $aSARAIVA, N. Z.
700 1 $aOLIVEIRA, L. Z.
773 $tAnatomia, Histologia, Embryologia$gv. 53, e13015, 2024.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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Registro Completo
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
07/11/2017 |
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Data da última atualização: |
22/10/2019 |
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Tipo da produção científica: |
Capítulo em Livro Técnico-Científico |
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Autoria: |
ASSIS, O. B. G. de; BRITTO, D. de. |
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Afiliação: |
ODILIO BENEDITO GARRIDO DE ASSIS, CNPDIA; DOUGLAS DE BRITTO, CPATSA. |
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Título: |
Coberturas comestíveis sobre frutas e hortaliças: fundamentos e prática. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
In: FERREIRA, M. D. (Ed.). Instrumentação pós-colheita em frutas e hortaliças. São Carlos, SP: Embrapa, 2017. |
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Páginas: |
cap. 2, 185-203. |
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Idioma: |
Português |
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Conteúdo: |
Tipos de coberturas; Formação da cobertura ou filme superficial; Características superficiais das frutas; Modos de aplicação das coberturas; Características da cobertura formada; Materiais empregados como coberturas; Nanotecnologia aplicada ao aprimoramento de revestimentos comestíveis |
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Palavras-Chave: |
Cobertura; Frutas comestíveis; Nanotecnologia; Postharvest; Revestimento comestivel. |
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Thesagro: |
Fruta; Hortaliça; Pós-colheita. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/203302/1/Coberturas-comestiveis-sobre-frutas-e-hortalicas.pdf
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Marc: |
LEADER 01014naa a2200241 a 4500
001 2079109
005 2019-10-22
008 2017 bl uuuu u00u1 u #d
100 1 $aASSIS, O. B. G. de
245 $aCoberturas comestíveis sobre frutas e hortaliças$bfundamentos e prática.
260 $c2017
300 $acap. 2, 185-203.
520 $aTipos de coberturas; Formação da cobertura ou filme superficial; Características superficiais das frutas; Modos de aplicação das coberturas; Características da cobertura formada; Materiais empregados como coberturas; Nanotecnologia aplicada ao aprimoramento de revestimentos comestíveis
650 $aFruta
650 $aHortaliça
650 $aPós-colheita
653 $aCobertura
653 $aFrutas comestíveis
653 $aNanotecnologia
653 $aPostharvest
653 $aRevestimento comestivel
700 1 $aBRITTO, D. de
773 $tIn: FERREIRA, M. D. (Ed.). Instrumentação pós-colheita em frutas e hortaliças. São Carlos, SP: Embrapa, 2017.
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